Portugal and Manchester United winger Nani has been ruled out of the World Cup with a left shoulder injury.
"After tests we conclude he is unfit to participate in the World Cup," the country's football federation said. Nani picked up the injury in training on Friday in Lisbon before flying to South Africa.
The winger had missed the squad's final home training session in Portugal on Saturday after falling while attempting an acrobatic shot, causing a traumatism in his shoulder.
During the flight to Johannesburg, he had been confident he would recover in time.
Nani's loss will be sorely felt by the 2006 semi-finalists who lost to runners-up France in Germany.
The winger had an excellent second half to the season with impressive performances in the Premier League and Champions League with Manchester United.
He will be replaced by Benfica's Ruben Amorim, the federation added.
Nani is the latest high-profile Premier League star who has been either ruled out or is very doubtful for the World Cup.
OUT
MICHAEL BALLACK, Germany and Chelsea
The German captain was ruled out of the finals with an ankle injury after a late challenge by Ghana's Kevin-Prince Boateng during Chelsea's FA Cup final win over Portsmouth in May.
RIO FERDINAND, England and Manchester United
The England captain will miss the finals after injuring his knee in his country's first major training session in South Africa last Friday.
MICHAEL ESSIEN, Ghana and Chelsea
The Ghana midfielder suffered a knee injury at the African Cup of Nations in January and has failed to recover in time.
JOHN OBI MIKEL, Nigeria and Chelsea
The midfielder was forced out of the tournament with a badly bruised ankle suffered in Nigeria's first full contact training session in South Africa on Friday.
JOSE BOSINGWA, Portugal and Chelsea
The full-back picked up a knee injury last October. He was expected to return last March but the club then said he required further surgery which ruled him out of South Africa.
DOUBTFUL
DIDIER DROGBA, Ivory Coast and Chelsea
The Ivory Coast's fearsome striker underwent successful surgery on an injured elbow on Saturday but remains a doubt for the finals. Drogba picked up the injury in a World Cup warm-up against Japan last Friday.
What is more, former Premier League stars David Beckham (England) and Lassana Diarra (France) are missing with injuries, with Arjen Robben (Netherlands) and Harry Kewell (Australia) doubtful.
